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What is the function of Restriction Endonucleases (e.g., EcoRI) in recombinant DNA technology?

Lernkarte aus dem Deck NEET UG High-Yield Review (Biology, Physics, Chemistry).

Antwort

They recognize specific palindromic nucleotide sequences in double-stranded DNA and cleave phosphodiester bonds, generating sticky or blunt ends.

Erklärung

Also known as molecular scissors, restriction enzymes allow target genes to be ligated into cloning vectors like plasmids (e.g., pBR322).

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