AdaAda

Home / Library / NEET UG High-Yield Review (Biology, Physics, Chemistry) / Synthetic flashcard

What is the function of Restriction Endonucleases (e.g., EcoRI) in recombinant DNA technology?

Study flashcard from the deck NEET UG High-Yield Review (Biology, Physics, Chemistry).

Answer

They recognize specific palindromic nucleotide sequences in double-stranded DNA and cleave phosphodiester bonds, generating sticky or blunt ends.

Explanation

Also known as molecular scissors, restriction enzymes allow target genes to be ligated into cloning vectors like plasmids (e.g., pBR322).

Synthetic content · Curated by Ada