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What is the function of Restriction Endonucleases (e.g., EcoRI) in recombinant DNA technology?

デッキ「NEET UG High-Yield Review (Biology, Physics, Chemistry)」の単語カード。

解答

They recognize specific palindromic nucleotide sequences in double-stranded DNA and cleave phosphodiester bonds, generating sticky or blunt ends.

解説

Also known as molecular scissors, restriction enzymes allow target genes to be ligated into cloning vectors like plasmids (e.g., pBR322).

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