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What is the function of Restriction Endonucleases (e.g., EcoRI) in recombinant DNA technology?

덱 'NEET UG High-Yield Review (Biology, Physics, Chemistry)'의 플래시카드.

정답

They recognize specific palindromic nucleotide sequences in double-stranded DNA and cleave phosphodiester bonds, generating sticky or blunt ends.

해설

Also known as molecular scissors, restriction enzymes allow target genes to be ligated into cloning vectors like plasmids (e.g., pBR322).

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